Video 3.
Protrusion of the APC’s plasma membrane toward a stalled Jurkat T cell centrosome. The Jurkat cell was transfected with GFP-Cen-2 to mark the centrosome, whereas the SEE-coated Raji B cell was labeled with PKH-26 to mark its plasma membrane. Images were acquired using a spinning disk confocal microscope (CSU-X1, Yokogawa Corporation of America; IX81, Olympus) and MetaMorph software, recorded at one frame every 15 s, and played back at 15 frames/s. Related to Fig. 4.