Video 6.
The dynamics of microtubules in a Jurkat T cell treated with 0.5 µM taxol and stimulated on an anti-CD3 antibody-coated coverslip surface. The Jurkat cell was transfected with 3×GFP-EMTB to mark microtubules and stimulated in the presence of 0.5 µM taxol on a coverslip surface coated with OKT3 antibody. Images were acquired using a spinning disk confocal microscope (CSU-X1, Yokogawa Corporation of America; IX81, Olympus) and MetaMorph software, recorded at 1 frame every 4 s, and played back at 15 frames/s. Related to Fig. S4.