Supplemental Figure 2 
1o Ab: MAB 1o Ab: Goat poly 1o Ab: BRIC TotCD59 anti-CD59 229 
2o Ab: Anti-2o Ab: Anti-goat 2o Ab: Anti-mouse IRDye IRDye 700 mouse IRDye 700 700 
1 2 1 21 2

Lane 1: Molecular wt marker 
Lane 2: Non diabetic urine (10X conc.) 
Supplemental Figure 2. Specificity of the anti-hCD59 mouse monoclonal antibody (MABTotCD59). 
A pool of human urine from 3 non-diabetic individuals (˜ 20 ml each) was lyophilized and reconstituted in PBS at a 10x concentration. Proteins were separated by SDS-PAGE and immunoblotted against two commercially available anti-CD59 antibodies (goat polyclonal anti-hCD59 and monoclonal anti-hCD59 BRIC-229) as well as MABTotCD59. All three antibodies recognized only one urine protein band (between the 15,000 and 21,000 Da molecular weight markers) characteristic of CD59. The inset shows an expanded view of the CD59 bands in each membrane. 

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