Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions

Files in this data supplement:

• Supporting Information: Figures S1-S5 (PDF, 5.5 MB)
• Figure S1: General strategy for genome editing across species (PDF, 714 KB)
• Figure S2: Diagram for the construction of a TALEN (PDF, 1 MB)
• Figure S3: Strategy for inserting FRT sites that flank rex-32 (PDF, 613 KB)
• Figure S4: in vitro DNA cleavage assays comparing the effectiveness of Cas9-crRNA: tracrRNA and Cas9-sgRNA complexes (PDF, 2.9 MB)
• Figure S5: Effect of Cas9 protein and sgRNA concentration on DNA cleavage efficiency in vitro (PDF, 680 KB)