Integration Profiling of Gene Function With Dense Maps of Transposon Integration

Files in this data supplement:

  • Supporting Information: Figures S1-S6, File S1, and Tables S1-S7 (PDF, 3 MB)
  • Figure S1: The percentage of cells with an insertion was measured during prolonged growth in liquid culture. (PDF, 400 KB)
  • Figure S2: The average nucleotide frequencies flanking the insertion sites were determined. (PDF, 469 KB)
  • Figure S3: The average nucleotide frequencies were determined for the positions on either side of the in vitro insertion sites. (PDF, 1 MB)
  • Figure S4: The average nucleotide frequencies were determined for the positions on either side of the in vivo insertion sites that occurred upstream of ORFs. (PDF, 491 KB)
  • Figure S5: The average nucleotide frequencies upstream and downstream of ORFs were determined. (PDF, 514 KB)
  • Figure S6: The average nucleotide frequencies upstream and downstream of ORFs in Saccharomyces cerevisiae were determined. (PDF, 760 KB)
  • File S1: Materials and Methods (PDF, 418 KB)
  • Table S1: ORFs designated by the consortium to be essential but had high integration densities (PDF, 425 KB)
  • Table S2: The levels of integration for each ORF of the genome (.xlsx, 972 KB)
  • Table S3: Test of deletion strains version 2.0 for presence of ORFs designated by the consortium to be nonessential (PDF, 754 KB)
  • Table S4: Chromosome coordinates of Hermes insertions (.txt, 5 MB)
  • Table S5: Strains of S. pombe used in this study (PDF, 511 KB)
  • Table S6: Plasmids (PDF, 403 KB)
  • Table S7: Oligonucleotides (PDF, 399 KB)