Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie S1. Co-localization of USE1 mRNA granules with the ER WT cells (BY4743Hα) expressing MS2L-tagged USE1 mRNA from its chromosomal locus were transformed with plasmids expressing MS2-CP-GFP(x3) (to label USE1 mRNA) and Sec63-RFP (to label the ER). Cells were grown to mid-log phase at 26°C on liquid synthetic selective medium and then shifted to the same medium lacking methionine for 1hr. Cells were placed on a thin agar block containing medium lacking methionine and visualized for 0.5hr using a DeltaVision imaging system. Images were processed by deconvolution. Note continuous association of the USE1 mRNA granule with the ER (either nuclear or cortical) throughout the course of the movie.
- Movie02 - Movie S2. Co-localization of SUC2 mRNA granules with the ER WT cells (BY4743Hα) expressing MS2L-tagged SUC2 mRNA from its chromosomal locus were transformed with plasmids expressing MS2-CP-GFP(x4) (to label SUC2 mRNA) and Sec63-RFP (to label the ER). Cells were grown to mid-log phase at 26°C on liquid synthetic selective medium and then shifted to low glucose (0.1%) medium lacking methionine for 1hr. Cells were placed on a thin agar block containing synthetic medium lacking methionine and visualized for 0.25hrs using a DeltaVision imaging system. Images were processed by deconvolution. Note continuous association of the SUC2 mRNA granules with the nuclear ER throughout the course of the movie.