Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - PKC activation increased retrograde actin flow rates in growth cones. FSM time lapse of F-actin dynamics in a growth cone injected with Alexa 594 phalloidin before and after 10 min in 100 nM PDBu. Scale bar: 5 μm; 5 sec interval; elapsed time: 2 min; playback: 8 frames per sec. This movie is from the growth cone in Figure 2A.
- Movie02 - Movie 2 Changes in actin dynamics during PKC activation. FSM time lapse of F-actin dynamics in a growth cone injected with Alexa 568 G-actin before and immediately after addition of 100 nM PDBu (at frame 47). Scale bar: 5 μm; 5 sec interval; elapsed time: 15 min; playback: 8 frames per sec. Note the slight focus shifts between frames 47-53 due to introduction of the drug. This movie is from the growth cone in Figure S4B.
- Movie03 - Movie 3 Filopodia merging during PKC activation. FSM time lapse of P domain F-actin dynamics in a growth cone injected with Alexa 594 phalloidin during 100 nM PDBu treatment. PDBu was added immediately before start of the movie. Scale bar: 5 μm; 10 sec interval; elapsed time: 5 min; playback: 8 frames per sec. This movie is from the growth cone in Figure S3.
- Movie04 - Movie 4 PKC activation did not increase retrograde flow in background of blebbistatin. FSM time lapse of F-actin dynamics in a growth cone injected with Alexa 594 phalloidin under control conditions, in blebbistatin (60 μM, 10 min), and after PDBu (100 nM, 10 min) addition in blebbistatin background. Scale bar: 5 μm; 5 sec interval; elapsed time: 2 min; playback: 8 frames per sec. This movie is from the growth cone in Figure 4A.