Video 2.
Dynamics of Ub and galectin3 in autophagy against transfected bead. (S1 and S2) NIH3T3 cells stably expressing mStrawberry (mStr)-Gal3 (magenta) and GFP-LC3 (green, S1) or mStr-Gal3 (magenta) and GFP-Ub (green, S2) were transfected with Effectene-coated latex beads for 30 min and then washed. Live cells were observed at 1-min intervals using a microscope (model IX81; Olympus) equipped with a xenon lamp and cooled charge-coupled device camera (CoolSnap HQ; Roper Scientific) under the control of MetaMorph software. Time is shown in h:min:s.