ERK1/2 
p-ERK1/2 
CRAMP 
CRAMP(mg/ml) 0 0.5 1 2 10 30
SupplementaryFig. 1 
K.Horibeet al. Figure S1Mouse 
(a)
(b)
SupplementaryFig. 2 
TRAP 
(+) 
mu
lt
in
u
clea
ted
c
ells/
w
ell
 
0 
200
800
1000 
400
600
M-CSF M-CSF
+ 
RANKL 
M-CSF 
+ 
RANKL 
+ 
CRAMP 
M-CSF
+ 
RANKL 
+ 
LL-37 
M-CSF M-CSF
+ 
RANKL 
M-CSF 
+ 
RANKL 
+ 
CRAMP 
M-CSF
+ 
RANKL 
+ 
LL-37 
Human  
TRAP
(+) 
mu
lt
in
u
clea
ted 
cell
s/
w
el
l ** 
** 
0 
200
100
50
150
M-CSF
+ 
RANKL 
M-CSF
+ 
RANKL 
+ 
CRAMP 
M-CSF
+ 
RANKL 
+ 
LL-37 
M-CSF
+ 
RANKL 
M-CSF
+ 
RANKL 
+ 
CRAMP 
M-CSF
+ 
RANKL 
+ 
LL-37 
K.Horibeet al. Figure S2