Functional Characterization of Core Components of the Bacillus subtilis Cyclic-Di-GMP Signaling Pathway

Supplemental material

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• Supplemental file 1 -

  Fig. S1, in vivo analysis of c-di-GMP production by putative DGCs in inducible-expression strains derived from wild-type B. subtilis

  Fig. S2, in vivo analysis of c-di-GMP production by putative DGCs in a background lacking all endogenous DGCs

  Fig. S3, in vivo detection of c-di-GMP in strains with a knockout of PdeH, a ci-di-GMP phosphodiesterase

  Fig. S4, in vitro detection of enzymatic activity of putative B. subtilis DGCs

  Fig. S5, measurement of binding affinities of c-di-GMP to YdaK and DgrA

  Fig. S6, B. subtilis biofilm formation is unaffected by alterations in c-di‐GMP signaling

  Fig. S7, swarm expansion assays of strains constitutively expressing YdaK or YkuI, potential c-di‐GMP receptors, in a background containing elevated c‐di‐GMP levels

  Fig. S8, PCR verification for dgc knockout mutants

  Table S1, B. subtilis strains

  Table S2, plasmids

  Table S3, primers

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