Detection of Escherichia coli O157 by Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) and Comparison to a Standard Culture Method

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    Comparison between the culture (ISO 16654:2001) and PNA-FISH results regarding E. coli O157:H7 detection in 60 food samples artificially contaminated, after a pre-enrichment in mTSB and BPW at 37°C (Table S1); partial alignment of 23S rDNA sequences for probe selection (Fig. S1); PNA-FISH outcome for heat-inactivated and viable E. coli CECT 4267 cells (Fig. S2).

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