Development of a Gene Knockout System Using Mobile Group II Introns (Targetron) and Genetic Disruption of Acid Production Pathways in Clostridium beijerinckii

Supplemental material

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• Supplemental file 1 -

  Strains, plasmids, and oligonucleotide primers used in this study (Table S1); schematic diagram for construction of the targetron plasmid pWY1 for gene knockout in C. beijerinckii (Fig. S1); dynamic transcriptional profiles of lactate dehydrogenase genes over the course of the batch fermentation process based on the RNA-Seq data published earlier (Fig. S2).

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