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Table S1. Real-time qRT-PCR analysis. Fig. S1. 6-FAM-labeled DNA probes used for DNaseI protection studies of the intergenic regions between plp and vah1 and rtxH and rtxB were obtained by PCR amplification using the primers in Table 2. Fig. S2. Real-time qRT-PCR was performed to determine the expression level of hlyU in the wild-type strain (M93Sm), hns mutant, and hns complement during exponential and stationary growth phases. Fig. S3. Growth curve and generation time of V. anguillarum wild-type (M93Sm), hns mutant, and hns complemented strains in LB20.
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