MrkD1P from Klebsiella pneumoniae Strain IA565 Allows for Coexistence with Pseudomonas aeruginosa and Protection from Protease-Mediated Biofilm Detachment

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    Fig. S1. Chymotrypsin has a negative effect on IApc35 and BC2 biofilms, and this effect is abrogated by either complementation with mrkD1P or the addition of antichymotrypsin. Fig. S2. Scanning electron micrographs illustrating the mean cell length of K. pneumoniae wild-type strain IA565, BC2-pBMC12, and BC2-pBMC13. Fig. S3. Effect of PAO1 supernatant on the monospecies biofilms of various strains of K. pneumoniae carrying mrkD1P or mrkD1C. Fig. S4. Strains of K. pneumoniae carrying various combinations of mrkD1C and mrkD1P are not defective for planktonic growth.

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