Video 3.
Dynamics of GFP-tubulin in a plated skeletal muscle FDB fiber. Single fibers were prepared from FDB muscles that had been injected with GFP-tubulin cDNA and electroporated 6 d earlier. Time-lapse sequences were recorded from the fibers on a TCS SP5 laser-scanning confocal microscope with a stage insert kept at 37°C. In this video focused near the surface of a fiber, frames were taken every second for 29 s. Arrowheads point to pockets of growing MTs. Additional technical parameters are listed in Table S1. Bar, 10 µm.