Video 9.
Live cell time-lapse microscopy using a DeltaVision microscope system to analyze DNA-associated Hoechst dye (greyscale). Frames were taken every 5 min. HeLa-EM2-11 mCherry–Topo IIα ΔChT cells were filmed while growing on glass-bottomed dishes after depletion of endogenous Topo IIα and release from concurrent synchrony in early S phase by double thymidine block. The absence of Doxycycline prevents induction of the exogenous mCherry–Topo IIα ΔChT. The cell undergoes aberrant chromosome condensation and failed chromosome segregation.