Identification of SPOR Domain Amino Acids Important for Septal Localization, Peptidoglycan Binding, and a Disulfide Bond in the Cell Division Protein FtsN

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Supplemental methods and results

  Table S1, strains

  Table S2, primers

  Table S3, localization of ^TTGFP-FtsN^SPOR in wild-type background

  Table S4, localization of ^TTGFP-FtsN^SPOR in an FtsN^SPOR null background

  Table S5, estimated distance between sulfur and α-carbons of C252 and C312 in 45 lowest energy structures of FtsN^SPOR

  Table S6, SPOR domain sequences with two cysteines

  Fig. S1, Western blot using anti-GFP antibody to document expression levels of select ^TTGFP-FtsN^SPOR mutant proteins

  Fig. S2, overproduction of His₆-FtsN^SPOR proteins

  Fig. S3, FtsN contains a disulfide bond

  Fig. S4, reduced abundance of FtsN in dsbA mutants grown in LB

  Fig. S5, efficient localization of ^TTGFP-DamX^SPOR does not require DsbA

  Fig. S6, modeling the effect of the disulfide bond on the structure of FtsN^SPOR reveals only minor changes

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