Video 2.
Lamellipodin regulates cell migration. This movie shows scrambled control shRNA (top) and Lpd-specific shRNA expressing Rat2 fibroblasts. Confluent monolayers were scratch-wounded and imaged every 5 min for 8 h by time-lapse phase contrast wide-field microscopy (IX81 [Olympus] with MetaMorph software [Molecular Devices]). Note that the Lpd knockdown Rat2 cells show slow residual cell migration by extending filopodia. Bar, 100 µm.