Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie 1 Dynamic localization of 2 nM GFP αE-catenin ABD (green) binding to phalloidin-stabilized Factin (20% Cy3-labeled, red). Images were acquired at 50 ms intervals. Movie plays at 20 frames per second. Scale bar 1 μm.
- Movie02 - Movie 2 Dynamic localization of 2 nM GFP αE-catenin ABD (green) binding to phalloidin-stabilized Factin (20% Cy3-labeled, red) in the presence of 0.5 μM dark αE-catenin ABD. Images were acquired every 50 ms during data acquisition. Movie plays at 20 frames per second. Scale bar 1 μm.
- Movie03 - Movie 3 Inhibition of Arp2/3 complex-mediated filament branching in the presence of GFP αE-catenin ABD. Actin filaments were polymerized and nucleated in the presence of 1 μM Mg-ATP-actin (10% Cy3), 50 nM Arp2/3, 100 nM SCARVCA, and 0-1 μM GFP αE-catenin ABD. Images were acquired every 10 sec during data acquisition. Movie plays at 15 frames per second. Scale bar 10 μm.
- Movie04 - Movie 4 Inhibition of Arp2/3 complex-mediated filament branching in the presence of full length GFP αE-catenin. Actin filaments were polymerized and nucleated in the presence of 1 μM Mg-ATPactin (10% Cy3 labeled), 50 nM Arp2/3 and 100 nM SCARVCA in the absence (right panel) or presence (left panel) of 2 μM full length GFP αE-catenin. Images were acquired every 10 sec during data acquisition. Movie plays at 15 frames per second. Scale bar 10 μm.
- Movie05 - Movie 5 Inhibition of cofilin severing in the presence of αE-catenin ABD. ADP-actin filaments (20% Cy3, red) were immobilized and disassembled by 75 nM ybbr-Atto488-hCofilin (green) in the absence (right panel) or presence (left panel) of 2 μM αE-catenin ABD. Images were acquired every 15 sec during data acquisition. Movie plays at 15 frames per second. Scale bar 5 μm.