Fig. 6. Polypeptide synthesis time course in Neurospora crassa cell-free extracts programmed with Met₉-AAP-globin-AAP-LUC transcripts. Transcripts specifying Met₉-AAP_m-globin-AAP_w-LUC (A and B) or Met₉-AAP_w-globin-AAP_m-LUC (C and D) were translated in extracts in low (A and C) or high Arg (B and D). Edeine was added at 2 min (arrow), and 10-m l aliquots of extracts were removed at the indicated time points for analysis by SDS/PAGE (see text). Arrowhead N indicates the intermediate Met₉-AAP; I, the polypeptide-intermediate Met₉-AAP-globin-AAP; F. the full-length polypeptide.