Fig. 8. (A) Growth curves of lamin and MAD2 siRNA-treated cells. 1.5 per 10⁵ viable HeLa cells were plated 72 h after transfection and were counted on days 4-7. Lamin-transfected cultures were confluent by day 6. (B) Microscopy image (´ 63) of a MAD2 knockdown HaCaT cell showing apoptotic bodies by DAPI stain (blue) and the absence of a discreet nuclear envelope by indirect immunofluorescence staining with antinuclear pore (Nup 358) antisera (red). (C and D). Attempted cytokinesis by MAD2 knockdown HeLa cells stained with DAPI (´ 63).