Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts

Video 1.
MT aster formation in crude interphase X. laevis egg extracts. Demembranated sperm was added to a crude interphase X. laevis egg extract containing Alexa fluor 488–labeled tubulin and the hydrophobic dye DiIC₁₈. The formation of MT asters (Alexa fluor 488 fluorescence; shown in white) was followed over time by spinning-disk confocal microscopy. The images were acquired at 20-s intervals for 15 min. The video is shown at 10 frames per second. Still images of this video were used for Fig. 1 A. Bar, 30 µm.