Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts

Video 3.
ER tubule extension toward the minus end of MTs. Demembranated sperm was added to a crude interphase X. laevis egg extract containing Alexa fluor 488–labeled tubulin and the hydrophobic dye DiIC₁₈. The samples were immediately imaged. Shown is the DiIC₁₈ fluorescence (in white). The arrowheads point to tubules extending toward the minus end of MTs. Time-lapsed images were acquired by spinning-disk confocal microscopy at 2-s intervals for 2 min. The video is shown at four frames per second. Bar, 5 µm.