Video 2.
CP110 regulates the length of the centriolar MTs in S2 cells. Movies show electron tomograms of centrioles in longitudinal orientation in S2 cells treated with GFP (control, left) and CP110 (right) RNAi. Tilt series of 150-nm thick sections were collected from −55° to 55° with 1° angular increment in a transmission electron microscope (TECNAI T12; FEI) at 13,000× using SerialEM (Mastronarde, 2005). Tomograms were reconstructed by R-weighted back projection with the user interphase eTomo and rendered three-dimensional using the IMOD package (Kremer et al., 1996). Centrioles were oriented along their longitudinal axis. Each movie frame in the tomogram is a projection of 10 individual slices. Note how long singlet and doublet MT extensions can be seen in the CP110-depleted centriole. Bar, 100 nm.