Video 2.
LC3-associated phagosomes persist for prolonged time periods. GFP-Atg8/LC3 (green)–expressing macrophages showing Atg8/LC3 translocation to zymosan-containing phagosomes. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (SP5; Leica). Frames were taken every 8 min for 4 h.