Supplemental Digital Content 3. PCR and SBE primers used for UGT genotyping.

Gene	Primer name	Sequence	Amplicon size	PCR Ta (°C)
UGT1A8	1A8 PCR-F 1A8 PCR-R rs1042597 SBE-R rs1042605 SBE-R rs1551285 PCR-F rs1551285 PCR-R rs1551285 SBE-R rs6431558 PCR-F rs6431558 PCR-R rs6431558 SBE-R	5'-AGTTCTCTCATGGCTCGCA 5'-TCACAATTCAGTAAGGAATCT 5'-ACCTTCTTCAAGATAGTGGCAA 5'-GGATAGTCCAAAACAAAGTC 5'-CACGGCAAGATCAGGTGAA 5'-AATTCTGCTGGCTTTATGCA 5'-GGTGATATAAAATCAGATATAC 5'-AGAAGGAAGCATTACTTAG 5'-TCTCATTAAAACTATTTCGC 5'-GGGTCTGGGACACTGCAC	941 bp - - 457 bp - 371 bp -	56 - - 56 - 51 -
UGT1A1	rs3755319 PCR-F rs3755319 PCR-R rs3755319 SBE-R	5'-TCTATGATCCTCTAAGCAC 5'-AAATTGTACTCATTCCACTG 5'-TGAGGAAATTCTGATGACTGA	292 bp -	51 -
UGT1A4	1A4 PCR-F 1A4 PCR-R rs6755571 SBE-F rs12468274 SBE-F rs2011404 SBE-F rs871514+rs13401281F rs871514+rs13401281R	5'-TTTGTCTTCCAATTACATGC 5'-TCACATAGAAWGTACCTCC 5'-CTCCTCCTCAGTGTCCAG 5'-AATGCTACTTCCTTTGATGTGGTT 5'-ATAGACCCCGTTAAGCTCTG 5'-AGATTTTATTTTGGCTTCTTTA 5'-CATCCATCCCACCTTCACA	659 bp - - - 429 bp	52 - - - 54

PCR, polymerase chain reaction; SBE, single base extension; Ta, annealing temperature; UGT, UDP-glucuronosyltransferase.

Underline refers to the modified base, W refers to the mixed base of A,T.