Video 1.
SKAP55 recruits to and co-migrates with SLP-76 downstream of TCR ligation. J14.SY cells expressing SLP-76.YFP (green) were transiently transfected with SKAP55.3×Flag.TRT (red) and plated on glass coverslips coated with 10 µg/ml OKT3 (anti-CD3). Frames were acquired every 2 s for 5 min by confocal microscopy (CSU-10 spinning-disc confocal [Yokogawa], Axiovert 200M stand [Carl Zeiss], 40× Plan-Neofluar oil-immersion objective lens [Carl Zeiss], and an ICCD camera [Stanford Photonics]). This video is related to Fig. 1.