Video 2.
3D rendering of a T-PC holding a FBT. RAW cells stably expressing GPI-GFP (white) were allowed to internalize FBTs (red) for 10 min. Cells were fixed with 4% PFA and external FBTs were immunolabeled (blue, not depicted). Confocal z-stacks were acquired 0.3 µm apart, deconvolved, and 3D rendered using Volocity software (PerkinElmer) to visualize a T-PC holding an FBT. Related to Fig. 1.