Filamentous morphology of bacteria delays the timing of phagosome morphogenesis in macrophages

Video 3.
Phagoyctosis of FBT by RAW cells stably expressing GFP-actin. Live-cell video microscopy showing phagocytosis of FBT (red) by RAW cells stably expressing GFP-actin (white). FBT attachment was synchronized and cells were moved to pre-warmed microscope stage (37°C/5% CO₂). 5 min after initial attachment, images were acquired every minute using a spinning disc confocal microscope and EM-CCD camera (Quorum Technologies Inc.) until complete internalization of the FBT. Images are merged, confocal z-planes. The movie is played at the rate of 5 frames per second. Related to Fig. 2.