Video 7.
Replication of long Lp02 filaments inside RAW macrophages. RAW cells were infected with Lp02. 10 h after initiation of phagocytosis, external bacteria were immunolabeled and cells were moved to a pre-warmed microscope stage. Infected cells were identified and intracellular survival of individual bacteria assessed by time-lapse live-cell video microscopy using a spinning disc confocal microscope and EM-CCD camera (Quorum Technologies Inc.). Long Lp02 filaments replicated inside RAW cells, leading to cell lysis and escape of bacillary progeny. Images were acquired every 45 min for 15 h and movie is played at 5 frames per second. Related to Fig. 9.