Video 8.
Destruction of intracellular filamentous Lp02 by RAW cells. RAW cells were infected with Lp02. 5 h after initial attachment external bacteria were immunolabeled and cells were moved to a pre-warmed microscope stage. Infected cells were identified and intracellular survival of individual bacteria assessed by time-lapse live-cell video microscopy using a spinning disc confocal microscope and EM-CCD camera (Quorum Technologies Inc.). Images shown depict an example of intracellular filamentous Lp02 being destroyed by RAW cells. Images were acquired every 45 min for 10 h and movie is played at 5 frames per second. Related to Fig. 9.