Segregation of Family A G Protein–Coupled Receptor Protomers in the Plasma Membrane

Files in this Data Supplement:

• Supplemental Data -

  Supplemental Figure 1 - A montage (z-stack) of images of beadinduced domains of His-beta2AR-venus

  Supplemental Figure 2 - Recruitment of cytosolic Nb80-GFP to bead-attached His-beta2AR-cerulean

  Supplemental Figure 3 - Recruitment of His-beta2AR-cerulean to IMAC beads without corecruitment of beta2AR-venus...

  Supplemental Figure 4 - Recruitment of His-C-mu-OR to IMAC beads

  Supplemental Figure 5 - IMAC beads that recruit His-beta2AR-cerulean have capacity to recruit additional (His-beta2AR-venus) protomers

  Supplemental Figure 6 - beta2AR-venus fluorescence in bead-apposed (Ibead) and surrounding (Ino bead) regions of the plasma membrane

  Supplemental Figure 7 - Recruitment of untagged GABA(B) and metabotropic glutamate receptors to IMAC beads

  Supplemental Figure 8 - The structure of BG-649-PEG-biotin

• Movie 1 -

  Supplemental Movie 1 - IMAC beads recruit His-beta2AR-venus

• Movie 2 -

  Supplemental Movie 2 - Recruitment of cytosolic Venus-arrestin3 to bead-associated His-beta2ARcerulean after stimulation with 10 muM isoproterenol

• Movie 3 -

  Supplemental Movie 3 - Recruitment of cytosolic Nb80-GFP to bead-associated His-beta2ARcerulean after stimulation with 10 muM isoproterenol

• Movie 4 -

  Supplemental Movie 4 - IMAC beads recruit His-beta2AR-cerulean but do not corecruit beta2ARvenus

• Movie 5 -

  Supplemental Movie 5 - Segregation of His-beta2AR-cerulean and SNAP-beta2AR-venus by mixed IMAC and sAV beads