Comprehensive Cell-specific Protein Analysis in Early and Late Pollen Development from Diploid Microsporocytes to Pollen Tube Growth

Supplemental Data

Files in this Data Supplement:

  • Supplementary Figure and Tables - Suppl. Figure 1 Relative abundance of proteins in 35 groups obtained by kmeans clustering. NSAF scores were averaged over three biological replicates and were normalized for each protein to represent their proportion of the total abundance over all eight stages. A, microsporocytes; B, meiotic cells; C, tetrads; D, microspores; F, binuclear pollen; G, desiccated pollen; H, pollen tubes.
  • supp table 1 - Suppl. Table 1 Peptide list. List of all peptides identified in all samples.
  • supp table 2 - Suppl. Table 2 Pollen proteins.
  • supp table 3 - Suppl. Table 3 Pollen and leaf proteins.
  • supp table 4 - Suppl. Table 4 PCA analysis of proteins from eight stages of pollen development.
  • supp table 1 - Suppl. Table 5 PCA analysis of proteins from eight stages of pollen development, leaves and roots.
  • supp table 6 - Suppl. Table 6 Kmean clustering of proteins from eight stages of pollen development.
  • supp table 7 - Suppl. Table 7 Functional clustering of proteins from stages of pollen development, leaves and roots.
  • Supplemental Table S8
  • supp table 10 - Suppl. Table 10 Arabidopsis genes and their corresponding tobacco protein homologues identified in this study.
  • supp table 11 - Literature survey of Arabidopsis genes affected in pollen development or pollen tube growth. Genes were blasted against tobacco proteins identified in this study.
  • supp table 12 - Suppl. Table 12 Peptides harboring post translational modifications.
  • Supplemental Table S9