Published January 20, 2014 // JCB vol. 204 no. 2 231-245
The Rockefeller University Press, doi: 10.1083/jcb.201307172

TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO–IKK supramolecular structures

• Video 1  Unstimulated human GFP-NEMO–expressing fibroblasts filmed with an UltraView spinning-disk confocal microscope with a 63× oil 1.4 NA objective lens.

• Video 2  Human GFP-NEMO–expressing fibroblasts stimulated with IL-1 (15 ng/ml).

• Video 3  Human GFP-NEMO-expressing fibroblasts stimulated with TNF (15 ng/ml).

• Video 4  TIRF microscopy of human GFP-NEMO–expressing fibroblasts stimulated with IL-1 (15 ng/ml).

• Video 5  TIRF microscopy of human GFP-NEMO–expressing fibroblasts stimulated with TNF (15 ng/ml).

• Video 6  FRAP experiment performed on human GFP-NEMO–expressing fibroblasts stimulated with IL-1 (15 ng/ml).

• Video 7  FRAP experiment performed on human GFP-NEMO–expressing fibroblasts stimulated with TNF (15 ng/ml).

• Supplemental material (PDF)

• Zipped files (zip) Contains the source code for computational methods.