Video 8.
Colocalization of microtubule subpopulations in differently coiled MBs. Related to Fig. 4 (A–C).Shown are 360° rotations of 3D reconstructions of confocal z-stack images of platelets triple immunostained with antibodies against acetylated α-tubulin (green), tyrosinated α-tubulin (red), and total α-tubulin (magenta). Individual stainings as well as merges are shown in the top images. Bars, 2 µm. The bottom images are generated from the different stainings after thresholding and skeletonizing of the MB. Images were acquired with a laser-scanning confocal microscope (LSM 710). (A) Resting platelet (stage 1; MB of 9.1 µm). (B) Platelet after 10 µM ATA (kinesin inhibitor) treatment for 3 min (beginning of coiling, stage 3; MB of 9 µm). (C) Platelet present in PRP from freshly drawn blood (coiled MB, stage 4; MB of 13.4 µm).