Regio- and Stereospecific Hydroxylation of Various Steroids at the 16α Position of the D Ring by the Streptomyces griseus Cytochrome P450 CYP154C3

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Equilibrium dissociation constants between CYP154C3 and the substrates (Fig. S1), determination of kinetic parameters of the CYP154C3-catalyzed hydroxylation of Δ⁴-androstene-3,17-dione (Fig. S2), spectroscopic data for 16α-hydroxytestosterone (Table S1), 16α-hydroxyprogesterone (Table S2), 16α-hydroxyadrenosterone (Table S4), and 16α-hydroxydeoxycorticosterone (Table S8) (in methanol-d⁴) and spectroscopic data for 16α-hydroxy-Δ⁴-androstene-3,17-dione (Table S3), 16α-hydroxy-1,4-androstadione-3,17-dione (Table S5), 16α-hydroxydehydroepiandrosterone (Table S6), and 16α-hydroxy-4-pregnane-3,11,20-trione (Table S7) (in chloroform-d₄).

  PDF, 460K