The Third Replicon of Members of the Burkholderia cepacia Complex, Plasmid pC3, Plays a Role in Stress Tolerance

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    Bacterial strains and plasmids used in this study (Table S1); oligonucleotides used in this study (Table S2); laboratory collection strains screened for pC3 presence (Table S3); WT Burkholderia cepacia complex (Bcc) strains show higher pathogenicity to G. mellonella than their pC3-null derivatives (Fig. S1); putative toxin-antitoxin gene sequences identified on pC3K56-2 using RASTA bacteria (Fig. S2); phenotypic microarray results showing carbon utilization phenotypes (Biolog plates PM1 [Table S4] and PM2a [Table S5]) or nitrogen utilization phenotypes (Biolog plate PM3b [Table S6]) that differ between wt strains and their pC3-null derivatives; visualization of undigested genomic DNA from wild-type B. cenocepacia K56-2 and its pC3-null derivative (Fig. S3); H111 and its pC3-null mutant grow equally well in minimal salts medium (Fig. S4).

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