Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol

Supplemental material

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• Supplemental file 1 -

  Primers used for introduction of His₆-tag, random mutagenesis, site-specific saturation mutagenesis, and site directed mutagenesis (Table S1); lipases used for sequence alignment (Table S2); oligonucleotides of specific mutations chosen by structure guided consensus method and hybridized into lipase T6 gene by ISOR (Table S3); representative phylogenetic tree dendogram constructed by the pairwise alignment method by ClustalW (Fig. S1); PRALINE multiple sequence alignment of G. stearothermophilus T6 lipase and the 9 homologous sequences (Fig. S2); SDS-PAGE of wild-type lipase T6 and mutants (Fig. S3).

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