Files in this Data Supplement:
Supplemental methods, phyogenetic reconstruction of NifH (Fig. S1) and AmoA (Fig. S2) deduced amino acid sequences recovered from Perpetual Spouter, primers, primer sequences, annealing temperatures, and corresponding references for the primers used in the present study (Table S1), dynamic ranges and detection limits of qPCR and qRT-PCR assays (Table S2), clone frequencies, accession numbers, and affiliations of nifH (Table S3) and amoA (Table S4) transcripts recovered in the present study, OTU frequencies, accession numbers, and affiliations of archaeal (Table S5) and bacterial (Table S6) 16S rRNA transcripts recovered in the present study, and rates of acetylene reduction in Azotobacter vinelandii (Table S7).
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