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Supplemental text; examples of force-distance curves recorded for a poly-l-lysine-coated cantilever (a) and a staphylococcal probe (S. aureus NCTC 8325-4) (b) on a glass surface taken in 10 mM potassium phosphate buffer (pH 7.0) under a maximal loading force of 3 nN (Fig. S1); retract force-distance curves for staphylococcal probes prepared with S. aureus NCTC 8325-4 (a), S. aureus NCTC 8325-4 Δpbp4 (b), S. aureus ATCC 12600 (c), and S. aureus ATCC 12600Δpbp4 (d) after different drying times (Fig. S2); replicates of retract force-distance curves recorded for a bacterial probe of S. aureus NCTC 8325-4 under a loading force of 3 nN at the same spot on a glass surface in 10 mM potassium phosphate buffer (pH 7.0) (Fig. S3); retract force-distance curves for a bacterial probe of S. aureus NCTC 8325-4 on a glass surface under loading forces Fld of 1, 3, 5, 7, and 9 nN in 10 mM potassium phosphate buffer (pH 7.0) (Fig. S4); hydrodynamic radii R0 measured by DLS (a) and polar radii rHeight Image determined using AFM imaging (b) for staphylococci harvested according to different protocols (Fig. S5).
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