Repression of Flagellar Genes in Exponential Phase by CsgD and CpxR, Two Crucial Modulators of Escherichia coli Biofilm Formation

Supplemental material

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  Table S1, primers

  Table S2, genes encoding transcriptional modulators slightly activated by RpoS in LB or M63 medium for which insertion mutants are available in the Keio collection of strain BW25113

  Fig. S1, expression of fliA under various growth conditions

  Fig. S2, csgD mutation affects swimming motility of E. coli in a temperature-independent manner

  Fig. S3, CsgD and CpxR affect neither rpoS transcription nor RpoS level and activity

  Fig. S4, detection of FliA in the csgD and cpxR mutant strains in exponential phase

  Fig. S5, negative regulation by csgD and cpxR of class 3 motility genes in exponential phase

  Fig. S6, CRP-cAMP activates fliA

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