Cdk1 Regulates the Temporal Recruitment of Telomerase and Cdc13-Stn1-Ten1 Complex for Telomere Replication

Supplemental material

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  • Supplemental file 1 -

    Fig. S1 (Putative Cdk1 phosphorylation sites in Stn1), S2 (Stn1 S250 antibody detection of stn1 recombinant protein), S3 (Telomere length measurement), S4 and S5 (Stn1 phosphorylation site mutations [S4] and phosphorylation [S5] in telomere elongation), S6 and S7 (STN1 T223 and S250 mutations [S6] and est2Δ [S7] in single-stranded telomere G-rich overhangs), S8 (Single-stranded telomere G-rich overhangs and DNA damage checkpoint), S9 (Stn1 phosphorylation and Stn1-Ten1 association), S10 and S13 (DNA content of yeast cultures used for ChIP experiments), S11 (Ten1 recruitment to telomere in stn1-T223A,S250A mutant), and S12 (STN1 T223 and S250 mutations and recruitment of CST complex and telomerase complex to telomeres); supplemental text; and Table S1 (Yeast strains)

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