Video 1.
Fluorescence anisotropy time-lapse video visualizing Eph receptor clustering kinetics. COS-7 cells were transfected with kdEphB2-3FKBP-mGFP and stimulated with AP20187 (250 nM) 1 min after start of epifluorescence time-lapse acquisition (frame 2). Images were acquired using an inverted microscope (IX81; Olympus) equipped with a MT20 illumination system. Acquisition at 1 frame/min. Fluorescence anisotropy is displayed according to the color code on the right. Bar, 10 µm.