Live imaging of prions reveals nascent PrP^Sc in cell-surface, raft-associated amyloid strings and webs

Video 1.
FL PrP^C dots are mobile on the surface of live GT1 cells. (Related to Fig. 5 B, a.) Cells were labeled at 8°C with 8B4 and fluorescent secondary Fabs, warmed gradually to 24°C, and analyzed by time-lapse wide field fluorescence microscopy (Axiovert 200M; Carl Zeiss; using an NA 1.3, 100× Plan Neofluar objective) at 400 ms/frame. Time of movie, 24 s; length, 60 frames. Many small dots (with integrated intensity of ∼0.3 arbitrary units; Fig. 5 C, b) move rapidly (mean mobility of 0.4 µm/s; Fig. 5 C, a)