Video 1.
Time-lapse imaging showing a fission event at MiD51-GFP foci in a COS-7 cell. COS-7 cells were transfected with MiD51-GFP (green) and stained for mitochondria with MitoTracker deep red (red). Time-lapse image analysis was performed using a laser-scanning confocal microscope (LSM 510; Carl Zeiss) equipped with a ConfoCor 3 system containing an avalanche photodiode detector using a 40× oil objective. Images were recorded every 5.5 s.