Supplemental Data
Files in this Data Supplement:
- supplemental figure S1 (.pdf, 1.7 MB) - Computational fit of single cell imaging traces.
- movie 1 (.avi, 7.7 MB) - HeLa cells were plated on a glass bottom dish and transfected with HIF-1alpha-EGFP 24 hours before imaging. Cells were then placed onto the microscope stage and imaged every 5 min as indicated in the main manuscript. Cells were initially imaged at 20.8%O2 for several frames before switching to 1%O2 for 20h. The movie is accelerated to 30 frames/sec.
- movie 2 (.avi, 2.8 MB) - HeLa cells were plated on a glass bottom dish and transfected with EGFP-HIF-2alpha 24 hours before imaging. Cells were then placed onto the microscope stage and imaged every 5 min as indicated in the main manuscript. Cells were initially imaged at 20.8%O2 for several frames before switching to 1%O2 for 15h. The movie is accelerated to 30 frames/sec.