Dual-indexing primers for 16S rRNA sequencing (319F/806R) on the MiSeq Platform -------------------------------------------------------- 319F (forward primer) PCR amplification primer sequence: -------------------------------------------------------- Field (space-delimited), description 1, 5’ Illumina Linker Sequence 2, Index 1 3, Heterogeneity Spacer 4, 319F Primer Sequence CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CCTAAACTACGG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TGCAGATCCAAC ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CCATCACATAGG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GTGGTATGGGAG T ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT ACTTTAAGGGTG T ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GAGCAACATCCT T ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TGTTGCGTTTCT GT ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT ATGTCCGACCAA GT ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT AGGTACGCAATT GT ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT ACAGCCACCCAT CGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TGTCTCGCAAGC CGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GAGGAGTAAAGC CGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GTTACGTGGTTG ATGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TACCGCCTCGGA ATGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CGTAAGATGCCT ATGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TACCGGCTTGCA TGCGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT ATCTAGTGGCAA TGCGA ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CCAGGGACTTCT TGCGT ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CACCTTACCTTA GAGTGG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT ATAGTTAGGGCT GAGTGG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GCACTTCATTTC GAGTGG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TTAACTGGAAGC CCTGTGG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CGCGGTTACTAA CCTGGAG ACTCCTACGGGAGGCAGCAG CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GAGACTATATGC CCTGGAG ACTCCTACGGGAGGCAGCAG -------------------------------------------------------- 806R (reverse primer) PCR amplification primer sequence: -------------------------------------------------------- Field (space-delimited), description 1, 3’ Illumina Linker Sequence 2, Index 2 3, Heterogeneity Spacer 4, 806R Primer Sequence AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CCTAAACTACGG GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT TGCAGATCCAAC GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CCATCACATAGG GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GTGGTATGGGAG A GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT ACTTTAAGGGTG A GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GAGCAACATCCT A GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT TGTTGCGTTTCT TC GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT ATGTCCGACCAA TC GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT AGGTACGCAATT TC GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT ACAGCCACCCAT CTA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT TGTCTCGCAAGC CTA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GAGGAGTAAAGC CTA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GTTACGTGGTTG GATA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT TACCGCCTCGGA GATA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CGTAAGATGCCT GATA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT TACCGGCTTGCA ACTCA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT ATCTAGTGGCAA ACTCA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CCAGGGACTTCT ACTCA GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CACCTTACCTTA TTCTCT GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT ATAGTTAGGGCT TTCTCT GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GCACTTCATTTC TTCTCT GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT TTAACTGGAAGC CACTTCT GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CGCGGTTACTAA CACTTCT GGACTACHVGGGTWTCTAAT AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GAGACTATATGC CACTTCT GGACTACHVGGGTWTCTAAT