Published March 3, 2014 // JCB vol. 204 no. 5 839-856
The Rockefeller University Press, doi: 10.1083/jcb.201306088

Twist1-induced dissemination preserves epithelial identity and requires E-cadherin

Video 9.
E-cad KD in Twist1-expressing tissue promoted collective epithelial migration. Representative DIC time-lapse movie of a CMV::rtTA;TRE-Twist1 organoid treated with lentiviral shRNA against E-cad (clone #2) and cultured in 3D Matrigel in medium with 2.5 nM FGF2 and doxycycline (corresponds to Fig. 10 G). Concurrent E-cad KD and Twist1 expression induced collective migration of long chains of cells. Video starts 7 h after doxycycline addition. Frames were collected every 20 min for 119 h (displayed at 24 frames/s) using a Cell Observer system with an AxioObserver Z1 and an AxioCam MRM camera (Carl Zeiss). Bar, 50 µm.