Files in this data supplement:
- Supporting Information: Figures S1-S8, Tables S1-S5, and Supporting Information References (PDF, 2 MB)
- Figure S1: Amino acid sequence alignment of Drosophila Nep1-Nep5 with Locusta migratoria ECE and Homo sapiens ECE-1, ECE-2 and neprilysin. (PDF, 1 MB)
- Figure S2: Neighbor-joining tree including LomECE and Drosophila Nep1-Nep5. (PDF, 344 KB)
- Figure S3: In situ hybridization results with sense probes for Nep1-5 in adult tissues revealed complete absence of signal. (PDF, 412 KB)
- Figure S4: Nep2 expression in the spermathecae. (PDF, 346 KB)
- Figure S5: RT PCRs (PDF, 354 KB)
- Figure S6: (A) The mean number of eggs laid by Control females or mates of Control males (grey line) or RNAi females or mates of RNAi males (black line) for the Nep1 and Nep4 alternative UAS-RNAi lines. (B) The mean hatchability (number of progeny/number of eggs) per female for the lines in part A. (PDF, 380 KB)
- Figure S7: The mean number of eggs laid by Control (grey line) or Nep2 RNAi females (black line) knocked down in one of three tissues (the CNS, spermathecae, or border cells) mated to WT males over a 10 day period. (PDF, 374 KB)
- Figure S8: The mean number of eggs laid by Control (grey line) or Nep1 RNAi females (black line) knocked down in one of two tissues (the CNS or the spermathecae) mated to WT males over a 10 day period. (PDF, 357 KB)
- Table S1: Gene Expression Profiles for Drosophila Neprilysin Family Genes in Reproductive Organs (PDF, 437 KB)
- Table S2: Summary of Neprilysin expression patterns. (PDF, 428 KB)
- Table S3: Knockdown and phenotype summary for Neprilysin genes. (PDF, 331 KB)
- Table S4: Nep cleavage sites in Drosophila neuropeptides. (PDF, 451 KB)
- Table S5: Tissue expression of neuropeptide candidates. (PDF, 330 KB)