Table 3. Theoretical primer specificity (in silico)

	Euryarchaeota (320)				Crenarchaeota (123)
Primer*	0	1	2	3	0	1	2	3
SDArch0333aS15	138^†	220	298	314	-	2^‡	3^§	27
SDArch0958aA19	126	294	300	303	57^¶	107	109	109

Specificity was determined from sequences 1,400 bp or longer. The number of sequences within each taxon is reported in parentheses. The number of phylotypes with the corresponding number of mismatches or fewer for each primer is given.

*Primers named according to OPD convention (1).

^{†Excludes all Halobacterium.
^{‡Solfolobus sulfataricus and Metalosphaera sedula.
^{§Acidianus ambivalens in addition to the above two species.
^{¶Inclusive of Desulfurococcales.
1. Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A. & Raskin, L. (1996) Appl. Environ. Microbiol. 62, 3557-3559.}}}}